{ "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:39:09 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 65, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "FrameKey-0-0-0": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "5.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -15, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 190, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "170df4ee-d45b-470f-8756-9d08e29b7d59", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 65, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:39:09 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 65, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "CFP/YFP", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "565/24", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "5.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 0, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 0, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "520", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 3257978, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-R2_002_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "190.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 57872.472, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-G_000.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 95, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-G", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:39:09 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 0, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -393.944, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-0-1": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "5.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -14, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 190, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "b69288d7-5634-4842-8b6c-e6e655a665ca", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 65, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:39:09 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 65, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "CFP/YFP", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "565/24", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "5.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 1, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 1, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "520", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 3258257, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-R2_002_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "190.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 57872.472, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-G_001.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 96, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-G", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:39:10 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 0, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -393.944, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-0-2": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "5.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -13, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 190, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "817be532-1552-49f8-bff5-f9136c2318b6", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 65, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:39:09 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 65, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "CFP/YFP", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "565/24", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "5.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 2, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 2, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "520", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 3258536, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-R2_002_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "190.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 57872.472, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-G_002.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 97, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-G", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:39:10 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 0, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -393.944, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-0-3": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "5.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -12, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 190, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "af59796f-6c07-4c16-9c7e-11f2b44d145c", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 65, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:39:09 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 65, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "CFP/YFP", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "565/24", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "5.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 3, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 3, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "520", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 3258815, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-R2_002_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "190.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 57872.472, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-G_003.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 98, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-G", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:39:10 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 0, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -393.944, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-0-4": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "5.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -11, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 190, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "c83a333a-c2c2-4f17-82fe-b7eba4351cba", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 65, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:39:09 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 65, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "CFP/YFP", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "565/24", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "5.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 4, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 4, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "520", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 3259092, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-R2_002_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "190.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 57872.472, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-G_004.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 99, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-G", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:39:11 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 0, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -393.944, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-0-5": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "5.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -10, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 190, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "6f60a00e-1f9c-493d-b2c9-78aadeaf7577", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 65, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:39:09 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 65, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "CFP/YFP", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "565/24", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "5.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 5, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 5, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "520", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 3259375, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-R2_002_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "190.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 57872.472, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-G_005.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 100, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-G", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:39:11 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 0, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -393.944, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-0-6": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "5.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -9, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 190, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "b011b229-117f-4a65-b66d-13bd9d0a6523", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 65, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:39:09 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 65, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "CFP/YFP", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "565/24", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "5.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 6, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 6, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "520", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 3259653, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-R2_002_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "190.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 57872.472, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-G_006.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 101, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-G", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:39:11 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 0, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -393.944, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-0-7": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "5.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -8, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 190, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "c0a7b209-537d-47f5-9884-298086e6f2d8", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 65, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:39:09 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 65, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "CFP/YFP", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "565/24", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "5.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 7, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 7, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "520", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 3259932, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-R2_002_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "190.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 57872.472, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-G_007.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 102, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-G", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:39:11 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 0, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -393.944, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-0-8": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "5.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -7, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 190, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "6fcec13e-b730-4a53-853e-3560516db63e", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 65, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:39:09 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 65, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "CFP/YFP", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "565/24", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "5.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 8, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 8, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "520", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 3260210, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-R2_002_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "190.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 57872.472, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-G_008.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 103, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-G", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:39:12 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 0, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -393.944, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-0-9": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "5.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -6, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 190, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "b5f0b15c-413c-4187-9ce7-01b3d60850ab", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 65, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:39:09 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 65, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "CFP/YFP", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "565/24", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "5.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 9, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 9, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "520", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 3260487, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-R2_002_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "190.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 57872.472, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-G_009.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 104, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-G", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:39:12 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 0, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -393.944, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-0-10": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "5.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -5, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 190, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "2247c9b2-a6fb-4b1b-b11d-8650615d83ae", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 65, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:39:09 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 65, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "CFP/YFP", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "565/24", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "5.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 10, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 10, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "520", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 3260765, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-R2_002_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "190.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 57872.472, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-G_010.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 105, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-G", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:39:12 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 0, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -393.944, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-0-11": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "5.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -4, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 190, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "8dbf3f9e-4f23-4ccf-b791-8ae9aaeaf582", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 65, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:39:09 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 65, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "CFP/YFP", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "565/24", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "5.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 11, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 11, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "520", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 3261041, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-R2_002_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "190.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 57872.472, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-G_011.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 106, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-G", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:39:13 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 0, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -393.944, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-0-12": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "5.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -3, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 190, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "25bb21a4-4b4f-4434-9d81-63aa1fc61e3c", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 65, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:39:09 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 65, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "CFP/YFP", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "565/24", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "5.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 12, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 12, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "520", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 3261319, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-R2_002_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "190.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 57872.472, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-G_012.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 107, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-G", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:39:13 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 0, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -393.944, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-0-13": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "5.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -2, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 190, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "f8b120f8-4aaf-4fd2-aef9-9329efa35c6b", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 65, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:39:09 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 65, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "CFP/YFP", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "565/24", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "5.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 13, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 13, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "520", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 3261596, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-R2_002_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "190.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 57872.472, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-G_013.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 108, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-G", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:39:13 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 0, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -393.944, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-0-14": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "5.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -1, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 190, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "aae0d243-a807-4ac0-ac44-76003c837915", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 65, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:39:09 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 65, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "CFP/YFP", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "565/24", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "5.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 14, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 14, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "520", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 3261873, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-R2_002_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "190.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 57872.472, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-G_014.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 109, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-G", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:39:13 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 0, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -393.944, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-0-15": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "5.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 0, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 190, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "a82b4055-b813-4eae-949a-8dda3f8bd203", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 65, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:39:09 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 65, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "CFP/YFP", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "565/24", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "5.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 15, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 15, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "520", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 3262152, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-R2_002_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "190.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 57872.472, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-G_015.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 110, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-G", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:39:14 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 0, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -393.944, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-0-16": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "5.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 1, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 190, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "70cbafc1-229c-4f59-a0c4-e1304b7baaab", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 65, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:39:09 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 65, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "CFP/YFP", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "565/24", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "5.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 16, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 16, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "520", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 3262431, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-R2_002_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "190.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 57872.472, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-G_016.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 111, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-G", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:39:14 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 0, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -393.944, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-0-17": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "5.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 2, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 190, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "f9e954c0-32c1-44cf-91a7-a141d5eca514", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 65, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:39:09 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 65, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "CFP/YFP", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "565/24", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "5.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 17, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 17, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "520", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 3262708, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-R2_002_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "190.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 57872.472, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-G_017.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 112, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-G", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:39:14 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 0, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -393.944, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-0-18": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "5.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 3, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 190, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "0bb14b45-c432-4cf4-a75f-58db5b6134a3", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 65, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:39:09 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 65, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "CFP/YFP", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "565/24", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "5.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 18, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 18, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "520", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 3262986, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-R2_002_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "190.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 57872.472, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-G_018.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 113, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-G", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:39:14 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 0, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -393.944, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-0-19": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "5.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 4, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 190, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "a0901544-059c-4a72-8e6f-6c108a0c978e", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 65, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:39:09 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 65, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "CFP/YFP", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "565/24", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "5.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 19, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 19, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "520", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 3263263, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-R2_002_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "190.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 57872.472, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-G_019.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 114, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-G", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:39:15 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 0, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -393.944, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-0-20": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "5.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 5, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 190, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "aaa3a7ab-125b-4de0-adb9-f993dbd532d1", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 65, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:39:09 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 65, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "CFP/YFP", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "565/24", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "5.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 20, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 20, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "520", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 3263539, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-R2_002_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "190.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 57872.472, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-G_020.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 115, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-G", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:39:15 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 0, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -393.944, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-0-21": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "5.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 6, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 190, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "18605001-f748-42d4-8b5f-005bdb7d717a", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 65, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:39:09 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 65, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "CFP/YFP", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "565/24", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "5.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 21, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 21, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "520", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 3263818, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-R2_002_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "190.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 57872.472, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-G_021.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 116, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-G", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:39:15 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 0, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -393.944, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-0-22": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "5.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 7, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 190, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "c50545bb-d0ed-4f31-9e3c-fb68572e4fb4", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 65, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:39:09 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 65, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "CFP/YFP", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "565/24", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "5.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 22, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 22, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "520", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 3264097, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-R2_002_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "190.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 57872.472, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-G_022.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 117, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-G", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:39:16 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 0, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -393.944, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-0-23": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "5.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 8, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 190, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "40d7ecb5-300b-477d-a26b-916431b4b675", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 65, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:39:09 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 65, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "CFP/YFP", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "565/24", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "5.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 23, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 23, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "520", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 3264378, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-R2_002_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "190.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 57872.472, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-G_023.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 118, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-G", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:39:16 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 0, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -393.944, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-0-24": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "5.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 9, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 190, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "331b0881-9eaa-4122-bdad-25fbba8f4566", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 65, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:39:09 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 65, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "CFP/YFP", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "565/24", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "5.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 24, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 24, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "520", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 3264655, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-R2_002_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "190.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 57872.472, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-G_024.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 119, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-G", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:39:16 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 0, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -393.944, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-0-25": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "5.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 10, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 190, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "87ca7aa9-cfb4-4a39-b5ce-a6af042c180f", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 65, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:39:09 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 65, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "CFP/YFP", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "565/24", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "5.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 25, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 25, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "520", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 3264935, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-R2_002_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "190.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 57872.472, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-G_025.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 120, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-G", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:39:16 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 0, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -393.944, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-0-26": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "5.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 11, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 190, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "f633c3da-7867-40bb-a25b-000b05d17ed3", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 65, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:39:09 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 65, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "CFP/YFP", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "565/24", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "5.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 26, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 26, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "520", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 3265214, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-R2_002_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "190.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 57872.472, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-G_026.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 121, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-G", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:39:17 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 0, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -393.944, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-0-27": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "5.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 12, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 190, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "25382401-b1dc-482d-8460-23470addbf95", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 65, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:39:09 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 65, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "CFP/YFP", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "565/24", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "5.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 27, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 27, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "520", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 3265491, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-R2_002_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "190.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 57872.472, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-G_027.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 122, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-G", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:39:17 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 0, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -393.944, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-0-28": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "5.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 13, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 190, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "797dbe08-32b6-446e-bc40-140325eb6c17", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 65, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:39:09 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 65, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "CFP/YFP", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "565/24", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "5.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 28, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 28, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "520", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 3265772, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-R2_002_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "190.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 57872.472, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-G_028.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 123, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-G", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:39:17 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 0, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -393.944, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-0-29": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "5.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 14, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 190, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "03777487-6c2d-4dd7-aaab-623b5ce8e5e9", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 65, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:39:09 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 65, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "CFP/YFP", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "565/24", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "5.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 29, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 29, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "520", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 3266049, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-R2_002_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "190.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 57872.472, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-G_029.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 124, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-G", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:39:18 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 0, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -393.944, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-0-30": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "5.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 15, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 190, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "3cf4566f-8faf-4b04-9569-89bb9f18bda7", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 65, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:39:09 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 65, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "CFP/YFP", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "565/24", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "5.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 30, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 30, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "520", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 3266327, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-R2_002_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "190.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 57872.472, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-G_030.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 125, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-G", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:39:18 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 0, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -393.944, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-1-0": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -15, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 80, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "83eb31bf-ba7c-490d-bf43-54059d31b517", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 65, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:39:09 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 65, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "585/11", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 0, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 0, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "555", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 3267256, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-R2_002_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "80.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 57872.472, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-T_000.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 95, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-T", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:39:19 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 1, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "5.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -393.944, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "5.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-1-1": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -14, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 80, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "6c2a95a9-0fe5-4552-a912-8d06b205e190", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 65, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:39:09 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 65, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "585/11", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 1, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 1, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "555", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 3267428, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-R2_002_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "80.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 57872.472, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-T_001.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 96, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-T", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:39:19 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 1, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "5.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -393.944, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "5.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-1-2": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -13, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 80, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "fe68b647-b0a9-473f-b932-f935bafdd55d", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 65, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:39:09 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 65, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "585/11", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 2, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 2, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "555", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 3267596, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-R2_002_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "80.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 57872.472, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-T_002.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 97, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-T", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:39:19 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 1, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "5.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -393.944, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "5.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-1-3": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -12, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 80, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "adbf1d87-91e4-42c9-820c-4a366328b224", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 65, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:39:09 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 65, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "585/11", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 3, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 3, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "555", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 3267766, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-R2_002_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "80.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 57872.472, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-T_003.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 98, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-T", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:39:19 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 1, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "5.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -393.944, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "5.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-1-4": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -11, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 80, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "c5f0cee0-ed7d-4485-acb7-9db1affb3363", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 65, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:39:09 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 65, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "585/11", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 4, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 4, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "555", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 3267936, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-R2_002_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "80.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 57872.472, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-T_004.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 99, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-T", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:39:19 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 1, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "5.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -393.944, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "5.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-1-5": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -10, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 80, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "f10c3cb0-a6a5-4d09-aa22-acdd1c2edf86", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 65, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:39:09 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 65, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "585/11", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 5, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 5, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "555", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 3268104, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-R2_002_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "80.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 57872.472, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-T_005.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 100, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-T", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:39:20 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 1, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "5.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -393.944, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "5.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-1-6": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -9, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 80, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "0e13850c-e0de-4a5c-ace1-5f76106ed942", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 65, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:39:09 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 65, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "585/11", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 6, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 6, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "555", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 3268270, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-R2_002_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "80.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 57872.472, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-T_006.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 101, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-T", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:39:20 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 1, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "5.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -393.944, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "5.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-1-7": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -8, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 80, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "8d8e12b5-26b0-42fa-bf5d-d311937e9de2", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 65, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:39:09 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 65, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "585/11", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 7, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 7, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "555", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 3268438, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-R2_002_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "80.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 57872.472, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-T_007.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 102, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-T", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:39:20 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 1, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "5.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -393.944, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "5.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-1-8": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -7, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 80, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "44ea967c-98ff-44dd-b18f-fbf225b47b62", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 65, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:39:09 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 65, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "585/11", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 8, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 8, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "555", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 3268606, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-R2_002_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "80.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 57872.472, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-T_008.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 103, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-T", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:39:20 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 1, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "5.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -393.944, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "5.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-1-9": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -6, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 80, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "c84b2466-932f-483e-8e9f-932a439fb231", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 65, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:39:09 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 65, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "585/11", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 9, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 9, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "555", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 3268773, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-R2_002_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "80.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 57872.472, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-T_009.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 104, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-T", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:39:20 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 1, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "5.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -393.944, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "5.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-1-10": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -5, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 80, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "4bf6232f-1f49-4132-a20a-aa6dfb7fc056", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 65, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:39:09 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 65, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "585/11", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 10, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 10, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "555", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 3268940, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-R2_002_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "80.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 57872.472, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-T_010.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 105, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-T", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:39:20 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 1, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "5.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -393.944, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "5.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-1-11": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -4, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 80, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "4e20b106-fe12-49aa-b469-2c1434db4bf3", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 65, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:39:09 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 65, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "585/11", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 11, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 11, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "555", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 3269109, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-R2_002_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "80.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 57872.472, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-T_011.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 106, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-T", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:39:21 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 1, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "5.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -393.944, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "5.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-1-12": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -3, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 80, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "f3826fb8-c8d5-4b9d-b0fb-b6f811a84f92", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 65, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:39:09 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 65, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "585/11", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 12, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 12, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "555", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 3269278, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-R2_002_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "80.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 57872.472, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-T_012.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 107, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-T", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:39:21 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 1, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "5.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -393.944, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "5.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-1-13": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -2, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 80, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "ac61bcc5-8a9e-4a23-b664-c467910425bc", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 65, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:39:09 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 65, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "585/11", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 13, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 13, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "555", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 3269446, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-R2_002_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "80.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 57872.472, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-T_013.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 108, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-T", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:39:21 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 1, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "5.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -393.944, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "5.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-1-14": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -1, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 80, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "598c5cc0-1b46-4f6a-907a-9eb03910f31a", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 65, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:39:09 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 65, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "585/11", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 14, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 14, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "555", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 3269613, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-R2_002_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "80.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 57872.472, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-T_014.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 109, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-T", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:39:21 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 1, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "5.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -393.944, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "5.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-1-15": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 0, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 80, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "bd477209-0444-4a63-97a6-74493ca4e5fd", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 65, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:39:09 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 65, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "585/11", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 15, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 15, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "555", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 3269779, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-R2_002_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "80.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 57872.472, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-T_015.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 110, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-T", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:39:21 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 1, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "5.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -393.944, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "5.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-1-16": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 1, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 80, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "626d3388-dbef-4ccc-a90a-c6457bb65a9e", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 65, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:39:09 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 65, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "585/11", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 16, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 16, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "555", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 3269947, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-R2_002_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "80.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 57872.472, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-T_016.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 111, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-T", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:39:21 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 1, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "5.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -393.944, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "5.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-1-17": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 2, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 80, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "4e9d37b5-66e3-485e-93c4-6df36aec980e", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 65, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:39:09 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 65, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "585/11", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 17, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 17, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "555", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 3270117, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-R2_002_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "80.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 57872.472, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-T_017.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 112, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-T", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:39:22 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 1, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "5.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -393.944, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "5.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-1-18": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 3, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 80, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "c2b4a69f-8705-4771-bf10-432d05c10b8b", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 65, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:39:09 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 65, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "585/11", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 18, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 18, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "555", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 3270287, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-R2_002_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "80.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 57872.472, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-T_018.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 113, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-T", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:39:22 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 1, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "5.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -393.944, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "5.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-1-19": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 4, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 80, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "fe8ab900-a453-4937-b965-7c5b7e447546", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 65, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:39:09 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 65, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "585/11", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 19, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 19, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "555", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 3270456, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-R2_002_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "80.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 57872.472, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-T_019.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 114, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-T", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:39:22 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 1, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "5.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -393.944, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "5.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-1-20": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 5, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 80, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "6f0581cb-8fbe-482c-8f0a-a1ac6d21f7b7", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 65, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:39:09 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 65, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "585/11", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 20, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 20, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "555", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 3270626, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-R2_002_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "80.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 57872.472, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-T_020.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 115, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-T", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:39:22 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 1, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "5.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -393.944, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "5.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-1-21": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 6, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 80, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "b464cfc4-65ba-4cc1-a851-74d20030e209", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 65, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:39:09 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 65, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "585/11", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 21, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 21, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "555", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 3270795, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-R2_002_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "80.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 57872.472, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-T_021.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 116, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-T", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:39:22 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 1, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "5.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -393.944, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "5.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-1-22": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 7, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 80, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "f0c0643e-b7af-4f52-99ca-2f992ef41f54", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 65, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:39:09 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 65, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "585/11", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 22, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 22, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "555", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 3270967, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-R2_002_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "80.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 57872.472, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-T_022.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 117, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-T", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:39:22 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 1, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "5.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -393.944, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "5.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-1-23": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 8, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 80, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "273678df-4b9f-4c4b-b4b4-029d54e2abc2", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 65, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:39:09 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 65, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "585/11", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 23, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 23, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "555", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 3271133, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-R2_002_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "80.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 57872.472, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-T_023.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 118, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-T", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:39:23 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 1, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "5.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -393.944, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "5.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-1-24": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 9, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 80, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "3d0156e4-62b4-411a-8320-e56457735620", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 65, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:39:09 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 65, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "585/11", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 24, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 24, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "555", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 3271316, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-R2_002_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "80.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 57872.472, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-T_024.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 119, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-T", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:39:23 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 1, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "5.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -393.944, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "5.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-1-25": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 10, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 80, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "e6858035-be64-4285-b043-e21253174931", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 65, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:39:09 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 65, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "585/11", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 25, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 25, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "555", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 3271483, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-R2_002_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "80.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 57872.472, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-T_025.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 120, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-T", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:39:23 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 1, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "5.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -393.944, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "5.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-1-26": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 11, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 80, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "ee35df80-9b92-41da-ae58-02bb76ef0cd9", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 65, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:39:09 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 65, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "585/11", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 26, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 26, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "555", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 3271651, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-R2_002_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "80.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 57872.472, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-T_026.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 121, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-T", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:39:23 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 1, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "5.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -393.944, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "5.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-1-27": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 12, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 80, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "468df441-eb4d-4684-a111-ef554296aed0", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 65, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:39:09 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 65, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "585/11", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 27, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 27, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "555", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 3271825, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-R2_002_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "80.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 57872.472, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-T_027.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 122, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-T", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:39:23 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 1, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "5.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -393.944, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "5.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-1-28": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 13, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 80, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "932d4871-416f-44f4-9333-6748e3482d14", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 65, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:39:09 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 65, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "585/11", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 28, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 28, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "555", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 3271995, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-R2_002_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "80.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 57872.472, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-T_028.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 123, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-T", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:39:23 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 1, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "5.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -393.944, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "5.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-1-29": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 14, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 80, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "0a4ba3b8-4b61-41ca-bad3-697cbb9a6ce8", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 65, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:39:09 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 65, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "585/11", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 29, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 29, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "555", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 3272165, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-R2_002_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "80.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 57872.472, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-T_029.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 124, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-T", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:39:24 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 1, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "5.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -393.944, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "5.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-1-30": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 15, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 80, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "35840642-2589-4e3f-934a-d6d266dcad33", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 65, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:39:09 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 65, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "585/11", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 30, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 30, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "555", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 3272337, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-R2_002_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "80.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 57872.472, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-T_030.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 125, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-T", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:39:24 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 1, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "5.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -393.944, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "5.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-2-0": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -15, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 180, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "123c4d8a-fdc3-4d66-83f7-556e3bd84fae", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 65, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:39:09 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 65, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "676/29", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 0, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 0, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 3273300, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-R2_002_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "180.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 57872.472, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-A_000.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 95, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-A", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:39:25 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 2, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -393.944, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-2-1": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -14, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 180, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "1c97a9d6-0250-482a-8a41-40057aefd150", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 65, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:39:09 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 65, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "676/29", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 1, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 1, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 3273571, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-R2_002_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "180.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 57872.472, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-A_001.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 96, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-A", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:39:25 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 2, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -393.944, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-2-2": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -13, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 180, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "224a1184-26f0-4296-8bb3-0e08ad2acc8a", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 65, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:39:09 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 65, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "676/29", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 2, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 2, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 3273839, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-R2_002_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "180.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 57872.472, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-A_002.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 97, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-A", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:39:25 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 2, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -393.944, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-2-3": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -12, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 180, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "0c6cf860-9848-40fd-ba70-a5b6c73f845e", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 65, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:39:09 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 65, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "676/29", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 3, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 3, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 3274105, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-R2_002_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "180.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 57872.472, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-A_003.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 98, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-A", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:39:26 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 2, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -393.944, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-2-4": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -11, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 180, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "597844c4-b685-496a-b1e4-2f64a05a6cb4", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 65, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:39:09 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 65, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "676/29", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 4, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 4, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 3274374, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-R2_002_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "180.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 57872.472, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-A_004.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 99, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-A", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:39:26 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 2, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -393.944, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-2-5": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -10, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 180, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "d9d6ed49-99d5-4560-a721-c84503eb84c4", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 65, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:39:09 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 65, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "676/29", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 5, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 5, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 3274643, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-R2_002_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "180.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 57872.472, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-A_005.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 100, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-A", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:39:26 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 2, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -393.944, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-2-6": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -9, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 180, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "acf72b32-ba37-4d9c-a7f8-a78a0ada3744", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 65, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:39:09 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 65, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "676/29", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 6, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 6, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 3274912, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-R2_002_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "180.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 57872.472, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-A_006.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 101, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-A", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:39:26 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 2, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -393.944, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-2-7": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -8, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 180, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "bd740b72-f61b-4978-8e7f-071d96c3193f", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 65, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:39:09 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 65, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "676/29", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 7, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 7, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 3275179, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-R2_002_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "180.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 57872.472, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-A_007.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 102, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-A", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:39:27 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 2, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -393.944, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-2-8": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -7, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 180, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "307cb917-0bc3-4ef2-b5ac-6385ff5ed6b6", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 65, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:39:09 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 65, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "676/29", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 8, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 8, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 3275447, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-R2_002_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "180.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 57872.472, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-A_008.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 103, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-A", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:39:27 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 2, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -393.944, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-2-9": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -6, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 180, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "cdaa6e56-69b7-4b60-82f4-a9cd0bcb18f3", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 65, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:39:09 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 65, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "676/29", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 9, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 9, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 3275714, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-R2_002_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "180.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 57872.472, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-A_009.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 104, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-A", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:39:27 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 2, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -393.944, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-2-10": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -5, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 180, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "d2f8119a-9a4d-423d-bcc0-d55556cb78f0", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 65, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:39:09 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 65, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "676/29", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 10, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 10, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 3275984, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-R2_002_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "180.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 57872.472, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-A_010.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 105, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-A", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:39:27 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 2, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -393.944, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-2-11": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -4, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 180, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "0683131d-7040-46f4-affa-c9d86981e8ed", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 65, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:39:09 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 65, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "676/29", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 11, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 11, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 3276254, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-R2_002_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "180.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 57872.472, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-A_011.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 106, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-A", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:39:28 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 2, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -393.944, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-2-12": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -3, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 180, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "da9ebf3c-38ec-486c-87ff-e6bffd8f8748", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 65, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:39:09 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 65, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "676/29", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 12, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 12, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 3276523, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-R2_002_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "180.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 57872.472, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-A_012.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 107, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-A", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:39:28 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 2, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -393.944, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-2-13": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -2, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 180, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "31c2d020-4350-464e-9d21-8c5fa51ae82a", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 65, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:39:09 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 65, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "676/29", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 13, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 13, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 3276789, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-R2_002_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "180.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 57872.472, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-A_013.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 108, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-A", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:39:28 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 2, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -393.944, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-2-14": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -1, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 180, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "213bf28e-fc4a-46a8-a184-047a121a72ae", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 65, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:39:09 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 65, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "676/29", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 14, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 14, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 3277057, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-R2_002_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "180.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 57872.472, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-A_014.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 109, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-A", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:39:29 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 2, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -393.944, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-2-15": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 0, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 180, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "4c7861b6-b802-4d38-8862-c5179f3168d3", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 65, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:39:09 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 65, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "676/29", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 15, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 15, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 3277324, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-R2_002_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "180.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 57872.472, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-A_015.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 110, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-A", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:39:29 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 2, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -393.944, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-2-16": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 1, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 180, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "a5d0adf7-3ebc-43a4-8d69-669963f55577", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 65, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:39:09 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 65, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "676/29", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 16, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 16, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 3277596, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-R2_002_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "180.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 57872.472, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-A_016.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 111, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-A", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:39:29 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 2, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -393.944, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-2-17": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 2, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 180, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "7d19c225-1dd3-4daa-a373-476af98d4d19", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 65, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:39:09 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 65, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "676/29", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 17, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 17, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 3277864, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-R2_002_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "180.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 57872.472, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-A_017.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 112, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-A", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:39:29 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 2, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -393.944, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-2-18": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 3, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 180, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "1d3712f5-aa61-47e7-9f54-4239d9827612", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 65, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:39:09 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 65, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "676/29", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 18, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 18, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 3278132, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-R2_002_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "180.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 57872.472, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-A_018.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 113, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-A", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:39:30 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 2, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -393.944, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-2-19": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 4, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 180, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "28779d3c-70f5-4d1a-852a-31c2b7a219b2", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 65, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:39:09 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 65, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "676/29", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 19, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 19, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 3278399, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-R2_002_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "180.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 57872.472, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-A_019.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 114, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-A", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:39:30 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 2, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -393.944, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-2-20": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 5, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 180, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "067fe470-4763-43cd-b713-6388c1fdf279", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 65, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:39:09 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 65, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "676/29", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 20, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 20, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 3278672, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-R2_002_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "180.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 57872.472, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-A_020.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 115, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-A", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:39:30 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 2, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -393.944, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-2-21": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 6, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 180, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "a3aa40fc-43f7-44c4-831e-c00ca4f2d8ad", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 65, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:39:09 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 65, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "676/29", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 21, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 21, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 3278949, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-R2_002_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "180.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 57872.472, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-A_021.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 116, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-A", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:39:30 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 2, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -393.944, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-2-22": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 7, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 180, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "88c1880c-765a-4a70-9912-9f24e904a695", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 65, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:39:09 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 65, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "676/29", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 22, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 22, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 3279217, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-R2_002_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "180.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 57872.472, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-A_022.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 117, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-A", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:39:31 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 2, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -393.944, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-2-23": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 8, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 180, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "dfc4ce7a-d68d-4e4e-a34c-60265a9dfcac", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 65, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:39:09 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 65, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "676/29", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 23, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 23, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 3279484, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-R2_002_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "180.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 57872.472, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-A_023.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 118, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-A", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:39:31 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 2, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -393.944, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-2-24": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 9, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 180, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "fc784f86-0663-4d69-8190-d76a17440d26", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 65, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:39:09 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 65, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "676/29", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 24, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 24, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 3279750, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-R2_002_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "180.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 57872.472, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-A_024.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 119, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-A", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:39:31 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 2, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -393.944, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-2-25": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 10, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 180, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "9fc3e58d-b903-481c-a1e2-b524fd70b30d", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 65, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:39:09 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 65, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "676/29", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 25, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 25, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 3280031, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-R2_002_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "180.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 57872.472, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-A_025.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 120, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-A", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:39:32 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 2, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -393.944, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-2-26": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 11, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 180, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "ff5ab59f-b5bb-46f0-a667-5294388525eb", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 65, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:39:09 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 65, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "676/29", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 26, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 26, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 3280301, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-R2_002_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "180.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 57872.472, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-A_026.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 121, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-A", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:39:32 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 2, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -393.944, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-2-27": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 12, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 180, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "29d7caeb-4853-4020-b3e0-4b7448c38019", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 65, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:39:09 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 65, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "676/29", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 27, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 27, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 3280567, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-R2_002_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "180.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 57872.472, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-A_027.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 122, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-A", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:39:32 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 2, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -393.944, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-2-28": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 13, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 180, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "6b81cf3f-7822-48cb-ba78-c29737ed8c8b", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 65, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:39:09 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 65, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "676/29", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 28, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 28, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 3280834, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-R2_002_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "180.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 57872.472, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-A_028.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 123, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-A", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:39:32 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 2, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -393.944, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-2-29": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 14, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 180, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "515318d0-41e5-4b82-949e-de469624ca4a", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 65, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:39:09 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 65, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "676/29", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 29, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 29, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 3281105, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-R2_002_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "180.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 57872.472, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-A_029.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 124, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-A", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:39:33 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 2, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -393.944, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-2-30": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 15, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 180, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "bbd5fc45-d458-4ecf-b886-4423aac01127", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 65, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:39:09 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 65, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "676/29", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 30, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 30, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 3281374, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-R2_002_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "180.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 57872.472, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-A_030.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 125, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-A", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:39:33 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 2, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -393.944, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-3-0": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -15, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 260, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "632bf599-40b4-4073-8d72-65792809d36f", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 65, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:39:09 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 65, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "725/40", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 0, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 0, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 3282012, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-R2_002_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "260.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 57872.472, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-C_000.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 95, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-C", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:39:34 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 3, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -393.944, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-3-1": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -14, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 260, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "dd50455e-a3a3-488e-96e9-bff1a2f75e0a", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 65, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:39:09 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 65, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "725/40", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 1, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 1, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 3282367, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-R2_002_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "260.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 57872.472, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-C_001.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 96, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-C", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:39:34 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 3, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -393.944, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-3-2": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -13, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 260, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "cc1ab7b4-9d23-4dd3-be4b-07228743f391", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 65, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:39:09 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 65, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "725/40", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 2, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 2, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 3282715, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-R2_002_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "260.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 57872.472, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-C_002.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 97, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-C", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:39:34 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 3, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -393.944, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-3-3": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -12, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 260, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "a887b83c-e60d-4c5f-be64-c2c5277757ea", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 65, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:39:09 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 65, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "725/40", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 3, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 3, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 3283065, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-R2_002_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "260.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 57872.472, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-C_003.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 98, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-C", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:39:35 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 3, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -393.944, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-3-4": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -11, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 260, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "adc069b4-211e-48e3-89e0-7603f8daa037", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 65, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:39:09 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 65, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "725/40", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 4, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 4, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 3283412, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-R2_002_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "260.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 57872.472, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-C_004.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 99, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-C", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:39:35 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 3, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -393.944, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-3-5": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -10, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 260, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "41cb64fa-8efc-4b40-92d6-e747cbe10be1", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 65, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:39:09 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 65, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "725/40", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 5, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 5, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 3283760, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-R2_002_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "260.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 57872.472, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-C_005.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 100, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-C", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:39:35 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 3, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -393.944, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-3-6": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -9, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 260, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "859c70bd-46e3-46e4-a067-023bbd6e5200", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 65, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:39:09 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 65, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "725/40", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 6, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 6, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 3284116, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-R2_002_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "260.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 57872.472, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-C_006.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 101, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-C", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:39:36 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 3, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -393.944, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-3-7": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -8, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 260, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "dd451e94-c00b-4757-bdcb-dce15c09c1b6", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 65, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:39:09 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 65, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "725/40", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 7, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 7, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 3284467, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-R2_002_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "260.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 57872.472, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-C_007.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 102, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-C", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:39:36 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 3, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -393.944, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-3-8": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -7, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 260, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "f8d77d8b-5525-4665-bbc7-0b4fbdaf66fb", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 65, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:39:09 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 65, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "725/40", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 8, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 8, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 3284815, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-R2_002_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "260.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 57872.472, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-C_008.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 103, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-C", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:39:36 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 3, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -393.944, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-3-9": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -6, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 260, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "e0b2049e-6641-451f-9566-39c174c7dd68", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 65, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:39:09 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 65, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "725/40", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 9, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 9, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 3285178, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-R2_002_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "260.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 57872.472, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-C_009.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 104, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-C", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:39:37 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 3, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -393.944, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-3-10": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -5, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 260, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "5dac20c4-e54b-41ab-adc6-4122303f04db", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 65, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:39:09 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 65, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "725/40", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 10, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 10, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 3285528, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-R2_002_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "260.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 57872.472, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-C_010.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 105, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-C", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:39:37 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 3, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -393.944, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-3-11": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -4, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 260, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "609083c9-0ce7-4957-935a-db276d2e5b63", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 65, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:39:09 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 65, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "725/40", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 11, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 11, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 3285875, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-R2_002_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "260.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 57872.472, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-C_011.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 106, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-C", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:39:37 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 3, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -393.944, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-3-12": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -3, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 260, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "b56bf598-e0cc-4fd4-b9b9-08848ad39b8d", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 65, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:39:09 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 65, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "725/40", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 12, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 12, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 3286235, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-R2_002_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "260.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 57872.472, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-C_012.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 107, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-C", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:39:38 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 3, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -393.944, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-3-13": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -2, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 260, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "7544c47b-acee-4c62-bc3b-54163d572600", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 65, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:39:09 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 65, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "725/40", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 13, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 13, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 3286583, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-R2_002_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "260.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 57872.472, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-C_013.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 108, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-C", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:39:38 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 3, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -393.944, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-3-14": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -1, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 260, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "d430c833-bd0d-4e7d-8b57-69a85b5c4a8e", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 65, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:39:09 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 65, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "725/40", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 14, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 14, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 3286931, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-R2_002_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "260.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 57872.472, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-C_014.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 109, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-C", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:39:38 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 3, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -393.944, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-3-15": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 0, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 260, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "9bd7aca3-71a1-4784-8b0e-6e7450a3143a", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 65, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:39:09 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 65, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "725/40", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 15, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 15, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 3287302, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-R2_002_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "260.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 57872.472, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-C_015.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 110, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-C", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:39:39 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 3, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -393.944, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-3-16": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 1, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 260, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "9dfa4a3f-8b20-4751-8ffc-e24aaaa20097", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 65, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:39:09 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 65, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "725/40", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 16, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 16, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 3287658, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-R2_002_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "260.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 57872.472, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-C_016.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 111, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-C", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:39:39 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 3, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -393.944, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-3-17": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 2, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 260, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "02b00ba8-a45a-4bf6-b1e8-fce5efefa0f0", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 65, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:39:09 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 65, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "725/40", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 17, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 17, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 3288008, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-R2_002_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "260.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 57872.472, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-C_017.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 112, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-C", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:39:39 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 3, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -393.944, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-3-18": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 3, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 260, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "fc451469-b5f6-46b5-ae4c-a4250228836c", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 65, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:39:09 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 65, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "725/40", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 18, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 18, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 3288368, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-R2_002_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "260.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 57872.472, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-C_018.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 113, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-C", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:39:40 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 3, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -393.944, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-3-19": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 4, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 260, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "c5f81765-5494-4857-a38a-3dc5e93f48f4", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 65, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:39:09 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 65, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "725/40", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 19, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 19, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 3288725, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-R2_002_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "260.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 57872.472, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-C_019.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 114, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-C", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:39:40 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 3, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -393.944, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-3-20": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 5, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 260, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "6ed3f5e5-ac3a-4b88-b155-52912338c143", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 65, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:39:09 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 65, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "725/40", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 20, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 20, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 3289072, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-R2_002_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "260.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 57872.472, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-C_020.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 115, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-C", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:39:41 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 3, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -393.944, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-3-21": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 6, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 260, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "b675a3bb-cbd9-4059-abc3-d7b21a314371", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 65, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:39:09 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 65, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "725/40", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 21, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 21, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 3289421, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-R2_002_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "260.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 57872.472, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-C_021.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 116, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-C", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:39:41 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 3, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -393.944, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-3-22": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 7, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 260, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "e9f2a119-637d-47b4-9a97-a8c6a633b9e4", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 65, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:39:09 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 65, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "725/40", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 22, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 22, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 3289770, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-R2_002_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "260.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 57872.472, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-C_022.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 117, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-C", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:39:41 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 3, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -393.944, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-3-23": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 8, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 260, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "f353ed8a-6aeb-4a66-92a5-3e41d1691ffe", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 65, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:39:09 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 65, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "725/40", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 23, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 23, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 3290117, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-R2_002_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "260.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 57872.472, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-C_023.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 118, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-C", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:39:42 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 3, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -393.944, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-3-24": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 9, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 260, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "54ad86b9-ea1c-441a-ad3f-f4cb868772c8", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 65, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:39:09 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 65, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "725/40", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 24, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 24, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 3290465, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-R2_002_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "260.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 57872.472, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-C_024.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 119, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-C", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:39:42 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 3, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -393.944, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-3-25": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 10, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 260, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "af421270-e676-4a24-a980-b135a04d9abd", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 65, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:39:09 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 65, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "725/40", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 25, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 25, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 3290826, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-R2_002_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "260.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 57872.472, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-C_025.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 120, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-C", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:39:42 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 3, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -393.944, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-3-26": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 11, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 260, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "79ea7f5c-c534-43e6-83b4-87eff3f395c2", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 65, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:39:09 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 65, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "725/40", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 26, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 26, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 3291191, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-R2_002_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "260.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 57872.472, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-C_026.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 121, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-C", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:39:43 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 3, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -393.944, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-3-27": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 12, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 260, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "915451fa-2755-4bc2-a5ad-019551e0d2ce", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 65, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:39:09 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 65, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "725/40", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 27, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 27, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 3291539, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-R2_002_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "260.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 57872.472, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-C_027.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 122, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-C", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:39:43 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 3, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -393.944, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-3-28": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 13, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 260, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "66a78c56-3e9b-4876-9e27-f924e4eec1db", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 65, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:39:09 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 65, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "725/40", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 28, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 28, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 3291886, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-R2_002_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "260.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 57872.472, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-C_028.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 123, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-C", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:39:43 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 3, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -393.944, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-3-29": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 14, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 260, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "619712bc-eb75-43e8-9727-21435439f57f", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 65, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:39:09 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 65, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "725/40", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 29, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 29, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 3292234, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-R2_002_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "260.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 57872.472, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-C_029.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 124, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-C", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:39:44 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 3, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -393.944, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-3-30": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 15, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 260, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "7f3b4ba7-e9e1-4b88-86fd-fd4fbcf2f617", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 65, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:39:09 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 65, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "725/40", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 30, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 30, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 3292591, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-R2_002_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "260.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 57872.472, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-C_030.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 125, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-C", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:39:44 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 3, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -393.944, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-4-0": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -15, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 10, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "5984f9ac-e379-45d6-b6f5-fd5612f9333b", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 65, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:39:09 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 65, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "647/57", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 0, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 0, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "LudlShutter", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 3293750, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-R2_002_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "10.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 57872.472, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_P-DIC_000.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 95, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "P-DIC", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:39:45 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 4, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -393.944, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-4-1": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -14, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 10, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "2ed07493-5fee-4cfe-934a-313cfb35e6b6", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 65, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:39:09 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 65, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "647/57", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 1, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 1, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "LudlShutter", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 3294182, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-R2_002_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "10.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 57872.472, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_P-DIC_001.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 96, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "P-DIC", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:39:46 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 4, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -393.944, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-4-2": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -13, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 10, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "1c34bbe8-acf8-457b-99b7-6fe97ba59ddb", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 65, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:39:09 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 65, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "647/57", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 2, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 2, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "LudlShutter", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 3294613, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-R2_002_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "10.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 57872.472, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_P-DIC_002.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 97, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "P-DIC", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:39:46 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 4, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -393.944, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-4-3": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -12, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 10, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "71a27032-d9df-4bd2-9cb0-104a856e2ae7", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 65, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:39:09 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 65, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "647/57", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 3, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 3, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "LudlShutter", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 3295031, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-R2_002_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "10.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 57872.472, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_P-DIC_003.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 98, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "P-DIC", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:39:47 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 4, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -393.944, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-4-4": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -11, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 10, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "130d2081-894f-46ec-8d9a-fb5d300950d8", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 65, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:39:09 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 65, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "647/57", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 4, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 4, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "LudlShutter", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 3295445, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-R2_002_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "10.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 57872.472, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_P-DIC_004.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 99, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "P-DIC", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:39:47 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 4, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -393.944, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-4-5": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -10, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 10, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "2317167e-e1b0-4f82-abe6-76f66814981d", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 65, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:39:09 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 65, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "647/57", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 5, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 5, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "LudlShutter", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 3295895, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-R2_002_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "10.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 57872.472, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_P-DIC_005.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 100, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "P-DIC", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:39:47 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 4, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -393.944, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-4-6": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -9, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 10, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "6ff756a6-31c4-4690-897a-dda4efd18593", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 65, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:39:09 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 65, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "647/57", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 6, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 6, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "LudlShutter", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 3296342, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-R2_002_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "10.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 57872.472, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_P-DIC_006.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 101, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "P-DIC", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:39:48 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 4, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -393.944, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-4-7": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -8, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 10, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "615fe53e-38c6-4947-a9a2-5331b2bdf225", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 65, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:39:09 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 65, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "647/57", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 7, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 7, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "LudlShutter", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 3296773, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-R2_002_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "10.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 57872.472, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_P-DIC_007.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 102, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "P-DIC", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:39:48 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 4, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -393.944, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-4-8": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -7, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 10, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "8c30f61e-3add-4763-b84f-3e6d2df40245", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 65, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:39:09 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 65, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "647/57", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 8, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 8, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "LudlShutter", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 3297221, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-R2_002_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "10.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 57872.472, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_P-DIC_008.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 103, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "P-DIC", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:39:49 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 4, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -393.944, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-4-9": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -6, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 10, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "1e68fb40-1583-4602-9dcb-f5fcd2cc058e", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 65, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:39:09 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 65, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "647/57", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 9, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 9, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "LudlShutter", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 3297670, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-R2_002_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "10.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 57872.472, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_P-DIC_009.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 104, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "P-DIC", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:39:49 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 4, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -393.944, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-4-10": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -5, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 10, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "8be4c113-919a-4255-b6f9-59662173b5fb", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 65, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:39:09 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 65, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "647/57", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 10, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 10, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "LudlShutter", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 3298085, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-R2_002_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "10.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 57872.472, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_P-DIC_010.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 105, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "P-DIC", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:39:50 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 4, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -393.944, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-4-11": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -4, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 10, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "d3b90464-9823-48af-b05e-c992e15f1e38", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 65, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:39:09 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 65, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "647/57", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 11, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 11, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "LudlShutter", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 3298516, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-R2_002_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "10.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 57872.472, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_P-DIC_011.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 106, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "P-DIC", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:39:50 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 4, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -393.944, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-4-12": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -3, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 10, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "f137181b-3f50-4644-8f35-c7e0a43e8cfd", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 65, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:39:09 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 65, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "647/57", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 12, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 12, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "LudlShutter", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 3298933, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-R2_002_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "10.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 57872.472, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_P-DIC_012.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 107, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "P-DIC", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:39:50 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 4, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -393.944, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-4-13": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -2, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 10, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "302c23e1-726f-4268-a55b-a651d72c7fd8", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 65, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:39:09 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 65, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "647/57", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 13, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 13, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "LudlShutter", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 3299363, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-R2_002_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "10.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 57872.472, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_P-DIC_013.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 108, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "P-DIC", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:39:51 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 4, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -393.944, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-4-14": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -1, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 10, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "fd1fefeb-fae9-43b9-8846-609fad3a119d", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 65, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:39:09 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 65, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "647/57", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 14, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 14, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "LudlShutter", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 3299781, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-R2_002_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "10.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 57872.472, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_P-DIC_014.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 109, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "P-DIC", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:39:51 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 4, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -393.944, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-4-15": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 0, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 10, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "ac998303-7e2f-49d4-bb19-b8ef7b451daf", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 65, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:39:09 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 65, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "647/57", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 15, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 15, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "LudlShutter", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 3300213, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-R2_002_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "10.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 57872.472, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_P-DIC_015.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 110, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "P-DIC", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:39:52 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 4, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -393.944, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-4-16": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 1, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 10, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "473f2b24-346a-4451-a991-c6199f9ed37b", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 65, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:39:09 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 65, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "647/57", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 16, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 16, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "LudlShutter", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 3300670, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-R2_002_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "10.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 57872.472, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_P-DIC_016.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 111, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "P-DIC", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:39:52 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 4, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -393.944, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-4-17": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 2, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 10, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "1c4dd322-af6f-4325-8d13-5a01a80b9e85", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 65, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:39:09 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 65, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "647/57", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 17, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 17, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "LudlShutter", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 3301093, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-R2_002_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "10.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 57872.472, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_P-DIC_017.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 112, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "P-DIC", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:39:53 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 4, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -393.944, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-4-18": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 3, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 10, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "baf7fbfc-1faf-471b-adc0-f509e7386fc1", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 65, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:39:09 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 65, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "647/57", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 18, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 18, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "LudlShutter", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 3301540, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-R2_002_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "10.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 57872.472, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_P-DIC_018.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 113, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "P-DIC", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:39:53 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 4, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -393.944, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-4-19": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 4, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 10, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "34fcdd7a-0128-4681-8f68-5ba052907196", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 65, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:39:09 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 65, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "647/57", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 19, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 19, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "LudlShutter", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 3301971, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-R2_002_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "10.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 57872.472, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_P-DIC_019.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 114, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "P-DIC", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:39:53 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 4, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -393.944, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-4-20": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 5, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 10, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "45bf9906-440f-4814-8373-47399343670e", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 65, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:39:09 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 65, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "647/57", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 20, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 20, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "LudlShutter", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 3302420, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-R2_002_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "10.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 57872.472, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_P-DIC_020.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 115, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "P-DIC", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:39:54 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 4, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -393.944, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-4-21": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 6, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 10, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "d9088a22-8c8d-4255-a52f-9e5257e2b685", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 65, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:39:09 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 65, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "647/57", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 21, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 21, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "LudlShutter", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 3302851, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-R2_002_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "10.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 57872.472, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_P-DIC_021.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 116, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "P-DIC", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:39:54 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 4, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -393.944, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-4-22": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 7, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 10, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "552118c4-27a3-4618-9bba-e87c3eb88dd8", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 65, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:39:09 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 65, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "647/57", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 22, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 22, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "LudlShutter", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 3303292, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-R2_002_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "10.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 57872.472, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_P-DIC_022.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 117, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "P-DIC", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:39:55 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 4, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -393.944, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-4-23": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 8, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 10, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "32e6eba2-9c69-442a-93db-b8e6ef05cd8e", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 65, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:39:09 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 65, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "647/57", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 23, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 23, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "LudlShutter", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 3303716, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-R2_002_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "10.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 57872.472, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_P-DIC_023.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 118, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "P-DIC", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:39:55 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 4, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -393.944, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-4-24": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 9, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 10, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "9f24898b-8842-421d-a6e2-4cc91b39eec3", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 65, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:39:09 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 65, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "647/57", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 24, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 24, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "LudlShutter", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 3304148, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-R2_002_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "10.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 57872.472, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_P-DIC_024.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 119, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "P-DIC", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:39:56 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 4, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -393.944, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-4-25": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 10, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 10, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "b1d520bd-cea8-4e58-9f15-02eedf2bc5fa", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 65, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:39:09 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 65, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "647/57", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 25, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 25, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "LudlShutter", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 3304581, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-R2_002_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "10.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 57872.472, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_P-DIC_025.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 120, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "P-DIC", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:39:56 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 4, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -393.944, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-4-26": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 11, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 10, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "db5914f1-a278-4325-a3b3-f38b33886e48", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 65, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:39:09 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 65, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "647/57", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 26, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 26, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "LudlShutter", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 3305027, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-R2_002_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "10.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 57872.472, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_P-DIC_026.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 121, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "P-DIC", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:39:57 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 4, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -393.944, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-4-27": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 12, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 10, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "f5ac464f-c7d6-43b3-91ac-9c255ea98530", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 65, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:39:09 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 65, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "647/57", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 27, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 27, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "LudlShutter", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 3305443, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-R2_002_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "10.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 57872.472, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_P-DIC_027.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 122, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "P-DIC", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:39:57 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 4, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -393.944, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-4-28": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 13, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 10, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "90667954-8cff-4ec3-a3d7-460626d62b04", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 65, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:39:09 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 65, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "647/57", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 28, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 28, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "LudlShutter", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 3305874, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-R2_002_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "10.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 57872.472, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_P-DIC_028.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 123, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "P-DIC", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:39:57 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 4, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -393.944, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-4-29": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 14, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 10, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "c97696f2-a8d7-40af-9db9-655b27c39403", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 65, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:39:09 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 65, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "647/57", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 29, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 29, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "LudlShutter", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 3306290, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-R2_002_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "10.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 57872.472, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_P-DIC_029.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 124, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "P-DIC", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:39:58 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 4, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -393.944, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-4-30": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 15, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 10, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "06537030-5a1b-45d9-a2fa-204778df52fc", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 65, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:39:09 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 65, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "647/57", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 30, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 30, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "LudlShutter", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 3306725, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-R2_002_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "10.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 57872.472, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_P-DIC_030.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 125, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "P-DIC", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:39:58 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 4, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -393.944, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" } }