{ "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:01:37 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 20, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "FrameKey-0-0-0": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "5.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -15, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 190, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "ff752175-8e5a-4d04-9664-3556a2494d0c", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 20, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:01:37 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 20, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "CFP/YFP", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "565/24", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "5.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 0, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 0, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "520", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1005391, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_006_002", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "190.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 62968.584, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-G_000.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 95, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-G", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:01:37 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 0, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -74, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-0-1": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "5.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -14, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 190, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "02a11820-9440-4a03-a2a1-02b68b320c65", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 20, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:01:37 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 20, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "CFP/YFP", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "565/24", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "5.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 1, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 1, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "520", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1005669, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_006_002", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "190.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 62968.584, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-G_001.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 96, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-G", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:01:37 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 0, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -74, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-0-2": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "5.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -13, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 190, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "5ab65635-efbf-4d6e-9f2e-2e7545082932", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 20, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:01:37 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 20, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "CFP/YFP", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "565/24", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "5.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 2, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 2, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "520", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1005952, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_006_002", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "190.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 62968.584, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-G_002.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 97, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-G", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:01:37 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 0, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -74, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-0-3": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "5.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -12, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 190, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "ffac18cc-58a9-43e6-bdd4-69b1a452d274", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 20, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:01:37 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 20, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "CFP/YFP", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "565/24", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "5.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 3, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 3, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "520", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1006231, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_006_002", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "190.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 62968.584, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-G_003.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 98, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-G", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:01:38 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 0, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -74, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-0-4": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "5.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -11, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 190, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "adc60be3-ff52-4ead-b2ec-787d97c735f3", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 20, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:01:37 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 20, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "CFP/YFP", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "565/24", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "5.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 4, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 4, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "520", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1006508, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_006_002", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "190.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 62968.584, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-G_004.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 99, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-G", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:01:38 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 0, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -74, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-0-5": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "5.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -10, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 190, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "76fadb9a-4202-45a7-997c-f5d199926af6", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 20, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:01:37 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 20, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "CFP/YFP", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "565/24", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "5.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 5, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 5, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "520", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1006785, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_006_002", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "190.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 62968.584, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-G_005.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 100, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-G", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:01:38 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 0, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -74, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-0-6": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "5.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -9, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 190, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "8bf84fc6-e977-49ec-b91f-1292c23f2c57", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 20, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:01:37 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 20, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "CFP/YFP", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "565/24", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "5.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 6, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 6, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "520", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1007062, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_006_002", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "190.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 62968.584, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-G_006.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 101, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-G", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:01:39 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 0, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -74, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-0-7": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "5.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -8, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 190, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "a29a0439-8000-4dae-be18-0b352e8b9d38", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 20, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:01:37 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 20, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "CFP/YFP", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "565/24", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "5.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 7, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 7, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "520", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1007341, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_006_002", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "190.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 62968.584, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-G_007.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 102, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-G", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:01:39 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 0, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -74, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-0-8": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "5.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -7, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 190, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "e5fc5dc6-cad6-4563-bbdc-259b896ae606", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 20, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:01:37 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 20, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "CFP/YFP", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "565/24", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "5.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 8, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 8, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "520", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1007625, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_006_002", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "190.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 62968.584, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-G_008.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 103, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-G", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:01:39 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 0, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -74, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-0-9": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "5.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -6, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 190, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "b84394fb-c31b-423a-a6aa-0bed1125a15c", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 20, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:01:37 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 20, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "CFP/YFP", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "565/24", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "5.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 9, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 9, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "520", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1007905, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_006_002", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "190.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 62968.584, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-G_009.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 104, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-G", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:01:39 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 0, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -74, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-0-10": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "5.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -5, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 190, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "e3b6da11-beef-4fea-8195-1944d963d711", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 20, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:01:37 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 20, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "CFP/YFP", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "565/24", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "5.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 10, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 10, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "520", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1008187, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_006_002", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "190.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 62968.584, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-G_010.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 105, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-G", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:01:40 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 0, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -74, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-0-11": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "5.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -4, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 190, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "afd02b79-e693-4366-96c2-34c98cb9d158", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 20, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:01:37 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 20, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "CFP/YFP", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "565/24", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "5.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 11, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 11, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "520", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1008465, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_006_002", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "190.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 62968.584, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-G_011.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 106, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-G", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:01:40 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 0, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -74, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-0-12": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "5.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -3, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 190, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "6954448f-4c3c-4bef-8914-b24b0ab8cab0", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 20, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:01:37 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 20, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "CFP/YFP", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "565/24", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "5.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 12, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 12, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "520", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1008742, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_006_002", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "190.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 62968.584, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-G_012.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 107, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-G", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:01:40 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 0, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -74, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-0-13": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "5.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -2, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 190, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "bbba0e3f-ecbf-400e-8a52-c1fb67ff7984", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 20, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:01:37 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 20, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "CFP/YFP", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "565/24", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "5.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 13, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 13, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "520", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1009022, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_006_002", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "190.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 62968.584, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-G_013.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 108, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-G", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:01:41 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 0, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -74, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-0-14": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "5.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -1, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 190, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "20d5d51c-c90b-4996-b16b-d380a3e573f6", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 20, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:01:37 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 20, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "CFP/YFP", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "565/24", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "5.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 14, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 14, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "520", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1009311, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_006_002", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "190.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 62968.584, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-G_014.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 109, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-G", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:01:41 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 0, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -74, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-0-15": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "5.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 0, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 190, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "95699f00-4566-4afc-ba1b-c5bce5bc20a7", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 20, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:01:37 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 20, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "CFP/YFP", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "565/24", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "5.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 15, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 15, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "520", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1009593, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_006_002", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "190.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 62968.584, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-G_015.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 110, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-G", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:01:41 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 0, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -74, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-0-16": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "5.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 1, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 190, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "98f7137c-61bd-4229-ba8f-2d33a88e2bb1", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 20, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:01:37 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 20, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "CFP/YFP", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "565/24", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "5.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 16, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 16, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "520", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1009870, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_006_002", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "190.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 62968.584, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-G_016.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 111, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-G", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:01:41 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 0, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -74, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-0-17": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "5.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 2, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 190, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "ea9d21f3-869d-414a-bbc2-446adb0ed638", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 20, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:01:37 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 20, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "CFP/YFP", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "565/24", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "5.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 17, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 17, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "520", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1010146, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_006_002", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "190.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 62968.584, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-G_017.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 112, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-G", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:01:42 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 0, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -74, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-0-18": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "5.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 3, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 190, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "29135b64-f0ff-4e5b-8488-c22b2ffd4f96", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 20, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:01:37 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 20, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "CFP/YFP", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "565/24", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "5.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 18, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 18, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "520", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1010424, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_006_002", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "190.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 62968.584, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-G_018.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 113, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-G", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:01:42 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 0, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -74, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-0-19": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "5.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 4, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 190, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "6179c9c2-7371-420e-8679-0541ca8e0779", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 20, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:01:37 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 20, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "CFP/YFP", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "565/24", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "5.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 19, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 19, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "520", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1010704, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_006_002", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "190.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 62968.584, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-G_019.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 114, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-G", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:01:42 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 0, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -74, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-0-20": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "5.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 5, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 190, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "bef8a121-cb4e-44d9-bfae-40e5b65137b2", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 20, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:01:37 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 20, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "CFP/YFP", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "565/24", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "5.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 20, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 20, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "520", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1010981, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_006_002", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "190.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 62968.584, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-G_020.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 115, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-G", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:01:42 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 0, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -74, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-0-21": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "5.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 6, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 190, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "894be2c9-31b7-46e8-a73a-c98aa1de2bf0", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 20, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:01:37 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 20, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "CFP/YFP", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "565/24", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "5.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 21, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 21, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "520", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1011258, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_006_002", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "190.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 62968.584, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-G_021.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 116, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-G", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:01:43 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 0, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -74, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-0-22": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "5.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 7, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 190, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "20addea3-7581-4139-98dc-eab27dfeca0a", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 20, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:01:37 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 20, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "CFP/YFP", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "565/24", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "5.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 22, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 22, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "520", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1011536, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_006_002", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "190.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 62968.584, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-G_022.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 117, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-G", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:01:43 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 0, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -74, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-0-23": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "5.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 8, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 190, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "f9097a61-ac01-46d4-8ece-1ab97340fd8b", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 20, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:01:37 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 20, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "CFP/YFP", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "565/24", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "5.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 23, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 23, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "520", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1011814, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_006_002", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "190.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 62968.584, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-G_023.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 118, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-G", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:01:43 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 0, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -74, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-0-24": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "5.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 9, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 190, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "01fe86ee-ddad-4fe2-bc13-1d6b2154143b", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 20, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:01:37 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 20, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "CFP/YFP", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "565/24", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "5.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 24, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 24, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "520", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1012094, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_006_002", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "190.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 62968.584, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-G_024.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 119, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-G", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:01:44 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 0, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -74, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-0-25": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "5.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 10, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 190, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "b3a2e794-93fe-499c-a7ab-41c5ac1a2342", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 20, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:01:37 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 20, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "CFP/YFP", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "565/24", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "5.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 25, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 25, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "520", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1012371, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_006_002", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "190.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 62968.584, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-G_025.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 120, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-G", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:01:44 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 0, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -74, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-0-26": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "5.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 11, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 190, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "eed95f28-3a32-4899-a1b6-72fd6e7212b4", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 20, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:01:37 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 20, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "CFP/YFP", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "565/24", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "5.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 26, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 26, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "520", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1012650, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_006_002", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "190.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 62968.584, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-G_026.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 121, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-G", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:01:44 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 0, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -74, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-0-27": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "5.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 12, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 190, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "52356283-8f92-4c2d-816e-d32daa898fc1", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 20, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:01:37 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 20, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "CFP/YFP", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "565/24", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "5.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 27, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 27, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "520", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1012945, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_006_002", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "190.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 62968.584, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-G_027.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 122, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-G", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:01:44 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 0, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -74, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-0-28": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "5.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 13, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 190, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "a79c0362-4e1c-4413-9f00-0e733a85bc5e", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 20, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:01:37 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 20, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "CFP/YFP", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "565/24", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "5.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 28, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 28, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "520", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1013223, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_006_002", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "190.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 62968.584, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-G_028.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 123, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-G", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:01:45 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 0, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -74, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-0-29": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "5.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 14, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 190, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "daa3fbca-97e3-460c-bef8-9b0b6950753f", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 20, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:01:37 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 20, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "CFP/YFP", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "565/24", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "5.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 29, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 29, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "520", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1013500, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_006_002", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "190.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 62968.584, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-G_029.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 124, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-G", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:01:45 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 0, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -74, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-0-30": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "5.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 15, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 190, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "d3b5b904-0357-4c4d-b7ef-9e2623212daa", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 20, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:01:37 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 20, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "CFP/YFP", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "565/24", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "5.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 30, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 30, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "520", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1013788, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_006_002", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "190.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 62968.584, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-G_030.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 125, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-G", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:01:45 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 0, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -74, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-1-0": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -15, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 80, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "01d43b37-db9c-44e8-9221-9c57fdd679d7", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 20, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:01:37 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 20, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "585/11", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 0, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 0, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "555", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1014732, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_006_002", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "80.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 62968.584, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-T_000.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 95, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-T", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:01:46 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 1, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "5.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -74, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "5.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-1-1": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -14, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 80, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "ee950e58-54a2-402c-8fc1-939f7685b580", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 20, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:01:37 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 20, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "585/11", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 1, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 1, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "555", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1014912, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_006_002", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "80.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 62968.584, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-T_001.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 96, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-T", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:01:46 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 1, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "5.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -74, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "5.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-1-2": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -13, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 80, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "a3877b72-050a-4939-a276-dc745af2cbfa", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 20, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:01:37 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 20, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "585/11", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 2, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 2, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "555", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1015079, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_006_002", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "80.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 62968.584, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-T_002.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 97, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-T", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:01:47 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 1, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "5.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -74, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "5.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-1-3": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -12, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 80, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "392348ba-52ac-4322-89a2-6ffecc93cbcb", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 20, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:01:37 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 20, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "585/11", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 3, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 3, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "555", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1015248, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_006_002", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "80.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 62968.584, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-T_003.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 98, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-T", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:01:47 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 1, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "5.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -74, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "5.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-1-4": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -11, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 80, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "c7a85c47-fda6-4efe-b1a0-313e54e11c6f", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 20, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:01:37 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 20, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "585/11", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 4, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 4, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "555", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1015416, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_006_002", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "80.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 62968.584, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-T_004.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 99, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-T", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:01:47 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 1, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "5.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -74, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "5.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-1-5": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -10, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 80, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "967f3709-bf87-4566-881c-104e4a2cf52a", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 20, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:01:37 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 20, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "585/11", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 5, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 5, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "555", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1015586, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_006_002", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "80.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 62968.584, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-T_005.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 100, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-T", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:01:47 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 1, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "5.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -74, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "5.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-1-6": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -9, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 80, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "ccdf3ab0-ede7-4ef7-a84e-54c7fbec6c23", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 20, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:01:37 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 20, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "585/11", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 6, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 6, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "555", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1015781, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_006_002", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "80.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 62968.584, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-T_006.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 101, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-T", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:01:47 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 1, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "5.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -74, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "5.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-1-7": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -8, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 80, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "91af3249-ff3f-4329-8d55-449e41a095ad", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 20, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:01:37 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 20, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "585/11", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 7, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 7, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "555", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1015947, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_006_002", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "80.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 62968.584, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-T_007.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 102, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-T", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:01:47 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 1, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "5.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -74, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "5.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-1-8": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -7, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 80, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "960baa30-ba70-45e1-88cf-de82736572d4", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 20, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:01:37 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 20, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "585/11", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 8, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 8, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "555", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1016116, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_006_002", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "80.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 62968.584, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-T_008.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 103, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-T", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:01:48 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 1, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "5.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -74, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "5.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-1-9": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -6, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 80, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "619d1c20-8ac0-42be-9d5d-2d0cbe2b2d01", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 20, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:01:37 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 20, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "585/11", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 9, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 9, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "555", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1016284, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_006_002", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "80.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 62968.584, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-T_009.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 104, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-T", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:01:48 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 1, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "5.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -74, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "5.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-1-10": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -5, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 80, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "cf84928f-b2ae-4404-ae29-420bba7a3320", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 20, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:01:37 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 20, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "585/11", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 10, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 10, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "555", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1016451, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_006_002", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "80.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 62968.584, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-T_010.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 105, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-T", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:01:48 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 1, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "5.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -74, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "5.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-1-11": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -4, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 80, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "cf2784a0-bd74-4a97-b7bc-65523f3b6c80", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 20, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:01:37 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 20, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "585/11", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 11, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 11, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "555", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1016620, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_006_002", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "80.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 62968.584, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-T_011.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 106, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-T", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:01:48 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 1, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "5.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -74, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "5.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-1-12": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -3, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 80, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "78a85424-217c-4951-820f-0f9773c5d8d4", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 20, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:01:37 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 20, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "585/11", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 12, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 12, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "555", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1016787, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_006_002", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "80.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 62968.584, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-T_012.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 107, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-T", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:01:48 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 1, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "5.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -74, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "5.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-1-13": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -2, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 80, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "a8c3cae5-1c39-4a13-8882-7165ab695b34", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 20, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:01:37 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 20, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "585/11", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 13, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 13, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "555", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1016955, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_006_002", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "80.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 62968.584, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-T_013.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 108, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-T", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:01:48 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 1, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "5.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -74, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "5.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-1-14": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -1, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 80, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "e8bed778-7dae-441c-8b06-c94c53a711cd", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 20, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:01:37 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 20, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "585/11", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 14, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 14, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "555", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1017125, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_006_002", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "80.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 62968.584, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-T_014.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 109, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-T", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:01:49 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 1, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "5.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -74, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "5.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-1-15": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 0, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 80, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "f7e5aca7-4b3a-4e61-9da4-419d1e1686f1", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 20, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:01:37 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 20, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "585/11", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 15, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 15, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "555", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1017293, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_006_002", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "80.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 62968.584, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-T_015.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 110, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-T", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:01:49 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 1, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "5.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -74, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "5.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-1-16": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 1, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 80, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "6d8b71a1-6de1-4d2d-b851-4eeeaf72316b", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 20, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:01:37 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 20, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "585/11", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 16, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 16, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "555", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1017461, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_006_002", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "80.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 62968.584, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-T_016.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 111, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-T", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:01:49 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 1, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "5.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -74, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "5.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-1-17": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 2, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 80, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "54698336-ba4a-4a5d-aeff-e518ee000cf6", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 20, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:01:37 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 20, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "585/11", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 17, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 17, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "555", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1017628, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_006_002", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "80.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 62968.584, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-T_017.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 112, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-T", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:01:49 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 1, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "5.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -74, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "5.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-1-18": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 3, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 80, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "d61142be-e1dc-40c4-9735-4ef39dd941e8", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 20, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:01:37 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 20, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "585/11", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 18, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 18, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "555", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1017810, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_006_002", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "80.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 62968.584, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-T_018.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 113, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-T", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:01:49 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 1, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "5.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -74, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "5.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-1-19": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 4, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 80, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "20c8d583-d53f-4d0e-80e6-0767ffe36825", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 20, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:01:37 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 20, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "585/11", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 19, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 19, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "555", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1018001, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_006_002", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "80.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 62968.584, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-T_019.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 114, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-T", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:01:49 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 1, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "5.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -74, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "5.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-1-20": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 5, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 80, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "1c74e302-a107-4f7a-a689-723504102b0b", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 20, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:01:37 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 20, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "585/11", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 20, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 20, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "555", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1018168, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_006_002", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "80.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 62968.584, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-T_020.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 115, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-T", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:01:50 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 1, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "5.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -74, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "5.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-1-21": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 6, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 80, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "0bfacf99-0baa-4935-9d73-f01489436983", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 20, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:01:37 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 20, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "585/11", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 21, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 21, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "555", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1018337, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_006_002", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "80.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 62968.584, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-T_021.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 116, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-T", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:01:50 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 1, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "5.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -74, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "5.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-1-22": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 7, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 80, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "978338b8-82dc-4cd1-a328-b8ab7f76efcd", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 20, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:01:37 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 20, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "585/11", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 22, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 22, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "555", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1018505, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_006_002", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "80.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 62968.584, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-T_022.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 117, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-T", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:01:50 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 1, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "5.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -74, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "5.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-1-23": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 8, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 80, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "713407be-7249-4017-a8c8-6d322a4bb8f9", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 20, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:01:37 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 20, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "585/11", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 23, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 23, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "555", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1018674, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_006_002", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "80.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 62968.584, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-T_023.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 118, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-T", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:01:50 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 1, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "5.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -74, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "5.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-1-24": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 9, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 80, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "3465c516-b52f-4302-80a2-c567f1bff76e", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 20, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:01:37 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 20, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "585/11", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 24, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 24, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "555", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1018843, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_006_002", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "80.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 62968.584, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-T_024.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 119, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-T", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:01:50 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 1, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "5.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -74, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "5.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-1-25": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 10, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 80, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "ffb9def8-9ec3-441f-83f4-afbae5649fd1", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 20, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:01:37 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 20, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "585/11", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 25, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 25, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "555", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1019011, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_006_002", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "80.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 62968.584, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-T_025.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 120, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-T", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:01:50 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 1, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "5.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -74, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "5.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-1-26": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 11, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 80, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "f94d5fd4-8d8a-4dca-ba75-3d0f4b70055e", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 20, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:01:37 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 20, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "585/11", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 26, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 26, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "555", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1019189, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_006_002", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "80.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 62968.584, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-T_026.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 121, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-T", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:01:51 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 1, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "5.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -74, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "5.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-1-27": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 12, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 80, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "75fd8358-ab57-4a94-bce8-8324709cd3ce", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 20, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:01:37 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 20, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "585/11", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 27, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 27, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "555", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1019358, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_006_002", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "80.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 62968.584, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-T_027.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 122, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-T", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:01:51 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 1, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "5.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -74, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "5.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-1-28": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 13, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 80, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "1fc27b0e-3fa4-41d7-ad17-609980549e03", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 20, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:01:37 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 20, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "585/11", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 28, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 28, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "555", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1019526, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_006_002", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "80.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 62968.584, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-T_028.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 123, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-T", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:01:51 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 1, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "5.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -74, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "5.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-1-29": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 14, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 80, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "41e5e5e5-453b-4a7e-9e9b-c0a137ae70cf", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 20, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:01:37 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 20, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "585/11", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 29, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 29, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "555", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1019694, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_006_002", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "80.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 62968.584, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-T_029.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 124, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-T", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:01:51 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 1, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "5.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -74, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "5.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-1-30": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 15, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 80, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "9aab3cc6-392d-4567-ba53-1eb431fecac0", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 20, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:01:37 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 20, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "585/11", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 30, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 30, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "555", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1019861, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_006_002", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "80.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 62968.584, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-T_030.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 125, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-T", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:01:51 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 1, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "5.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -74, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "5.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-2-0": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -15, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 180, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "66a7bf84-91d9-4ce2-b0bd-6094f265b4b9", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 20, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:01:37 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 20, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "676/29", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 0, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 0, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1020832, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_006_002", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "180.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 62968.584, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-A_000.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 95, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-A", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:01:52 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 2, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -74, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-2-1": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -14, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 180, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "3c60bd75-2c79-4f50-96e2-8ab4c3012809", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 20, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:01:37 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 20, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "676/29", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 1, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 1, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1021100, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_006_002", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "180.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 62968.584, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-A_001.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 96, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-A", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:01:53 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 2, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -74, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-2-2": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -13, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 180, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "1cc30489-34c9-411d-a7fe-2cdf0cb1d8a2", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 20, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:01:37 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 20, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "676/29", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 2, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 2, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1021369, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_006_002", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "180.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 62968.584, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-A_002.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 97, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-A", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:01:53 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 2, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -74, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-2-3": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -12, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 180, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "3db42bc6-0407-4f7a-b66a-9812f49122da", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 20, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:01:37 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 20, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "676/29", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 3, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 3, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1021636, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_006_002", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "180.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 62968.584, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-A_003.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 98, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-A", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:01:53 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 2, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -74, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-2-4": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -11, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 180, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "8eb95d1e-b7e0-44e4-81da-9c763bafc481", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 20, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:01:37 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 20, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "676/29", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 4, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 4, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1021905, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_006_002", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "180.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 62968.584, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-A_004.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 99, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-A", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:01:53 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 2, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -74, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-2-5": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -10, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 180, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "7770324b-a2db-432b-bce5-9e1fdb9ca99f", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 20, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:01:37 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 20, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "676/29", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 5, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 5, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1022173, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_006_002", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "180.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 62968.584, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-A_005.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 100, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-A", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:01:54 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 2, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -74, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-2-6": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -9, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 180, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "438b32ef-2d9b-4bdc-9c75-960ebe064c92", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 20, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:01:37 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 20, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "676/29", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 6, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 6, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1022451, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_006_002", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "180.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 62968.584, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-A_006.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 101, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-A", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:01:54 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 2, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -74, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-2-7": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -8, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 180, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "c5b312df-4e10-4d4b-966e-0af3d5856376", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 20, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:01:37 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 20, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "676/29", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 7, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 7, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1022721, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_006_002", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "180.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 62968.584, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-A_007.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 102, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-A", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:01:54 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 2, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -74, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-2-8": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -7, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 180, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "077457d1-0c69-4c2b-ba0d-393c9517c13d", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 20, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:01:37 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 20, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "676/29", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 8, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 8, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1022988, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_006_002", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "180.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 62968.584, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-A_008.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 103, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-A", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:01:54 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 2, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -74, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-2-9": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -6, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 180, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "c2bfa40d-af90-4eb1-bf84-7d6cd03ca22c", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 20, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:01:37 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 20, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "676/29", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 9, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 9, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1023257, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_006_002", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "180.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 62968.584, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-A_009.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 104, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-A", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:01:55 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 2, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -74, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-2-10": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -5, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 180, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "18034685-960f-4387-8292-b978d0e4cb24", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 20, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:01:37 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 20, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "676/29", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 10, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 10, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1023526, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_006_002", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "180.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 62968.584, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-A_010.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 105, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-A", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:01:55 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 2, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -74, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-2-11": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -4, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 180, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "117e24ca-39bf-4708-aee7-685ad0c5aeef", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 20, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:01:37 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 20, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "676/29", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 11, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 11, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1023799, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_006_002", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "180.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 62968.584, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-A_011.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 106, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-A", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:01:55 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 2, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -74, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-2-12": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -3, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 180, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "d557c157-0ff8-4690-ac10-2cf0684730f4", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 20, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:01:37 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 20, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "676/29", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 12, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 12, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1024084, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_006_002", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "180.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 62968.584, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-A_012.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 107, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-A", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:01:56 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 2, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -74, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-2-13": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -2, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 180, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "e2b84562-453b-4cbb-9d7e-4b6dfe2dc237", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 20, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:01:37 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 20, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "676/29", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 13, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 13, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1024352, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_006_002", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "180.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 62968.584, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-A_013.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 108, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-A", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:01:56 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 2, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -74, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-2-14": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -1, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 180, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "50147548-0165-43b4-8155-4c74e22dfc0e", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 20, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:01:37 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 20, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "676/29", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 14, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 14, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1024620, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_006_002", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "180.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 62968.584, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-A_014.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 109, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-A", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:01:56 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 2, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -74, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-2-15": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 0, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 180, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "07cdcb4f-e779-4bc0-a4fe-1ab0593edfc8", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 20, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:01:37 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 20, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "676/29", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 15, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 15, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1024887, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_006_002", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "180.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 62968.584, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-A_015.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 110, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-A", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:01:56 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 2, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -74, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-2-16": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 1, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 180, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "a776106c-4b6d-4dc9-85ec-6cdd7b4a159a", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 20, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:01:37 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 20, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "676/29", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 16, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 16, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1025154, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_006_002", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "180.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 62968.584, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-A_016.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 111, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-A", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:01:57 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 2, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -74, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-2-17": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 2, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 180, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "e24562f5-abc1-4eb6-96b8-cdea28fd7e9a", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 20, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:01:37 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 20, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "676/29", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 17, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 17, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1025424, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_006_002", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "180.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 62968.584, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-A_017.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 112, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-A", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:01:57 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 2, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -74, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-2-18": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 3, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 180, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "3bc46040-9362-4a8f-9f0c-183301b4c8f4", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 20, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:01:37 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 20, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "676/29", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 18, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 18, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1025695, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_006_002", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "180.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 62968.584, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-A_018.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 113, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-A", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:01:57 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 2, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -74, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-2-19": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 4, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 180, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "a176adee-c086-4a8d-b8c8-a2a6ed5b4659", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 20, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:01:37 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 20, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "676/29", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 19, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 19, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1025966, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_006_002", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "180.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 62968.584, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-A_019.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 114, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-A", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:01:57 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 2, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -74, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-2-20": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 5, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 180, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "299eeae3-5a83-420c-8932-87068f117a46", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 20, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:01:37 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 20, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "676/29", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 20, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 20, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1026232, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_006_002", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "180.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 62968.584, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-A_020.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 115, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-A", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:01:58 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 2, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -74, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-2-21": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 6, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 180, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "d73e6bdd-5d22-4db6-bd4f-20193bb2dbda", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 20, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:01:37 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 20, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "676/29", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 21, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 21, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1026500, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_006_002", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "180.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 62968.584, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-A_021.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 116, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-A", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:01:58 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 2, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -74, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-2-22": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 7, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 180, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "592a4dbe-0f68-435a-86ea-ab897cdc9a88", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 20, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:01:37 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 20, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "676/29", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 22, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 22, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1026769, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_006_002", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "180.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 62968.584, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-A_022.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 117, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-A", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:01:58 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 2, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -74, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-2-23": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 8, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 180, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "9470802d-feca-442b-a8ab-29d2dab186b8", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 20, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:01:37 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 20, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "676/29", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 23, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 23, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1027039, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_006_002", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "180.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 62968.584, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-A_023.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 118, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-A", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:01:59 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 2, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -74, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-2-24": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 9, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 180, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "763f705e-c742-40cb-9c31-eb5ff82ea357", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 20, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:01:37 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 20, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "676/29", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 24, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 24, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1027308, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_006_002", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "180.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 62968.584, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-A_024.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 119, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-A", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:01:59 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 2, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -74, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-2-25": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 10, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 180, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "09e2b838-0405-4938-b6a7-f220a0eb3cd5", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 20, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:01:37 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 20, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "676/29", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 25, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 25, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1027577, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_006_002", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "180.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 62968.584, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-A_025.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 120, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-A", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:01:59 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 2, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -74, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-2-26": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 11, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 180, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "e69a5f3f-1000-4efb-908b-fada406bb861", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 20, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:01:37 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 20, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "676/29", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 26, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 26, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1027848, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_006_002", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "180.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 62968.584, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-A_026.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 121, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-A", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:01:59 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 2, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -74, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-2-27": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 12, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 180, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "ef484ffe-85d2-45aa-89b6-fb8be70ddf07", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 20, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:01:37 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 20, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "676/29", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 27, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 27, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1028116, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_006_002", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "180.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 62968.584, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-A_027.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 122, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-A", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:02:00 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 2, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -74, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-2-28": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 13, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 180, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "5a71a8b9-a1a7-48bf-9d6d-a71d77be37d8", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 20, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:01:37 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 20, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "676/29", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 28, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 28, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1028387, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_006_002", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "180.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 62968.584, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-A_028.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 123, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-A", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:02:00 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 2, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -74, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-2-29": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 14, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 180, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "91a1e895-d09e-4ebf-8387-ea1b6cbef0da", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 20, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:01:37 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 20, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "676/29", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 29, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 29, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1028654, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_006_002", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "180.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 62968.584, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-A_029.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 124, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-A", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:02:00 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 2, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -74, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-2-30": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 15, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 180, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "61dce080-770a-43f8-a0f3-b34d5f8683f2", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 20, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:01:37 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 20, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "676/29", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 30, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 30, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1028922, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_006_002", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "180.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 62968.584, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-A_030.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 125, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-A", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:02:00 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 2, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -74, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-3-0": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -15, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 260, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "eab1edb6-6a44-46cb-89c8-69c5f865e715", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 20, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:01:37 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 20, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "725/40", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 0, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 0, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1029552, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_006_002", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "260.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 62968.584, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-C_000.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 95, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-C", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:02:01 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 3, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -74, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-3-1": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -14, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 260, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "f9fbeefd-e961-4acb-9e03-296a8e429a7f", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 20, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:01:37 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 20, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "725/40", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 1, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 1, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1029902, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_006_002", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "260.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 62968.584, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-C_001.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 96, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-C", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:02:01 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 3, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -74, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-3-2": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -13, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 260, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "f7762ea5-3a5f-4800-a9d5-a74e809b8a16", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 20, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:01:37 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 20, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "725/40", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 2, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 2, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1030250, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_006_002", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "260.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 62968.584, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-C_002.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 97, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-C", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:02:02 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 3, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -74, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-3-3": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -12, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 260, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "21f5e270-a073-4556-aeeb-3c8be73ad124", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 20, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:01:37 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 20, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "725/40", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 3, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 3, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1030598, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_006_002", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "260.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 62968.584, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-C_003.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 98, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-C", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:02:02 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 3, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -74, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-3-4": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -11, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 260, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "5fae6127-8ace-41da-a0a4-c385ed0e3b13", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 20, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:01:37 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 20, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "725/40", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 4, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 4, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1030946, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_006_002", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "260.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 62968.584, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-C_004.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 99, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-C", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:02:02 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 3, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -74, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-3-5": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -10, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 260, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "ed6d81f0-6320-43fb-b966-bffb25ba20e0", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 20, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:01:37 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 20, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "725/40", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 5, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 5, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1031293, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_006_002", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "260.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 62968.584, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-C_005.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 100, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-C", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:02:03 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 3, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -74, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-3-6": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -9, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 260, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "7a44043c-6a5b-43f3-b0be-be5b03596d5e", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 20, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:01:37 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 20, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "725/40", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 6, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 6, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1031641, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_006_002", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "260.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 62968.584, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-C_006.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 101, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-C", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:02:03 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 3, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -74, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-3-7": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -8, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 260, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "0621019d-e4d1-4067-b512-7f59dcb10c71", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 20, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:01:37 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 20, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "725/40", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 7, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 7, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1031989, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_006_002", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "260.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 62968.584, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-C_007.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 102, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-C", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:02:03 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 3, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -74, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-3-8": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -7, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 260, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "1b616695-4e03-4e23-a98a-891c64f23b68", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 20, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:01:37 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 20, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "725/40", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 8, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 8, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1032338, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_006_002", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "260.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 62968.584, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-C_008.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 103, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-C", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:02:04 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 3, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -74, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-3-9": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -6, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 260, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "30f4d0f5-c14a-4322-8436-8431be4a437a", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 20, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:01:37 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 20, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "725/40", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 9, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 9, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1032691, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_006_002", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "260.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 62968.584, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-C_009.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 104, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-C", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:02:04 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 3, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -74, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-3-10": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -5, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 260, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "f05ff88d-ba86-43c0-ab09-46ec44ab0081", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 20, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:01:37 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 20, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "725/40", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 10, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 10, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1033039, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_006_002", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "260.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 62968.584, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-C_010.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 105, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-C", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:02:05 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 3, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -74, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-3-11": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -4, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 260, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "5eb4844c-1e1f-4716-86f1-d22ce7dfebbb", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 20, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:01:37 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 20, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "725/40", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 11, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 11, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1033387, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_006_002", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "260.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 62968.584, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-C_011.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 106, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-C", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:02:05 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 3, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -74, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-3-12": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -3, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 260, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "d1d6d6e1-8b28-4cd2-a6d4-0f7be97e9e84", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 20, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:01:37 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 20, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "725/40", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 12, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 12, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1033734, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_006_002", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "260.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 62968.584, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-C_012.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 107, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-C", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:02:05 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 3, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -74, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-3-13": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -2, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 260, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "7ce2d749-9966-4a29-9f18-8071156b223e", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 20, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:01:37 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 20, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "725/40", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 13, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 13, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1034083, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_006_002", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "260.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 62968.584, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-C_013.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 108, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-C", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:02:06 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 3, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -74, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-3-14": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -1, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 260, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "02f4f644-a7c2-4554-984c-53094f7b3878", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 20, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:01:37 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 20, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "725/40", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 14, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 14, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1034430, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_006_002", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "260.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 62968.584, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-C_014.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 109, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-C", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:02:06 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 3, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -74, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-3-15": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 0, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 260, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "ef31fe03-779e-478c-9856-1d943cfb0b6d", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 20, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:01:37 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 20, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "725/40", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 15, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 15, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1034778, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_006_002", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "260.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 62968.584, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-C_015.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 110, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-C", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:02:06 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 3, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -74, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-3-16": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 1, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 260, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "1879c28f-c979-4041-9f48-fa16f4b558d7", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 20, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:01:37 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 20, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "725/40", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 16, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 16, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1035126, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_006_002", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "260.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 62968.584, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-C_016.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 111, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-C", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:02:07 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 3, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -74, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-3-17": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 2, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 260, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "03e5da20-e59b-4ed9-afe0-debe1b8478b3", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 20, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:01:37 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 20, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "725/40", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 17, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 17, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1035474, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_006_002", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "260.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 62968.584, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-C_017.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 112, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-C", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:02:07 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 3, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -74, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-3-18": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 3, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 260, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "63f99bc5-b103-4227-9816-5151dc430d1b", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 20, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:01:37 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 20, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "725/40", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 18, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 18, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1035823, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_006_002", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "260.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 62968.584, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-C_018.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 113, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-C", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:02:07 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 3, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -74, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-3-19": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 4, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 260, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "96e31470-e8a0-4e93-a9db-79cf46623ec0", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 20, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:01:37 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 20, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "725/40", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 19, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 19, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1036172, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_006_002", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "260.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 62968.584, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-C_019.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 114, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-C", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:02:08 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 3, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -74, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-3-20": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 5, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 260, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "b059c37f-b975-4df4-bdaa-182a150f7807", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 20, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:01:37 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 20, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "725/40", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 20, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 20, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1036519, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_006_002", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "260.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 62968.584, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-C_020.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 115, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-C", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:02:08 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 3, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -74, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-3-21": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 6, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 260, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "6df43a3d-00b8-4ca4-b346-6ee1ffed023b", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 20, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:01:37 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 20, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "725/40", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 21, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 21, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1036866, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_006_002", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "260.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 62968.584, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-C_021.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 116, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-C", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:02:08 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 3, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -74, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-3-22": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 7, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 260, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "668da715-d7fa-4de3-a22e-0554afe873e3", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 20, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:01:37 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 20, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "725/40", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 22, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 22, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1037213, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_006_002", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "260.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 62968.584, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-C_022.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 117, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-C", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:02:09 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 3, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -74, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-3-23": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 8, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 260, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "b78c612c-7104-42a2-b153-af1cf632b1a8", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 20, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:01:37 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 20, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "725/40", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 23, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 23, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1037561, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_006_002", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "260.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 62968.584, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-C_023.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 118, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-C", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:02:09 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 3, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -74, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-3-24": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 9, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 260, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "b441a783-a778-4635-9c32-52ead504b051", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 20, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:01:37 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 20, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "725/40", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 24, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 24, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1037909, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_006_002", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "260.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 62968.584, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-C_024.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 119, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-C", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:02:09 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 3, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -74, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-3-25": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 10, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 260, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "67bffffc-c3b9-42b3-91a7-e98ae9f87c25", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 20, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:01:37 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 20, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "725/40", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 25, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 25, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1038257, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_006_002", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "260.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 62968.584, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-C_025.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 120, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-C", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:02:10 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 3, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -74, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-3-26": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 11, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 260, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "92cf6acd-80b0-4265-a065-fae96a770acf", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 20, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:01:37 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 20, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "725/40", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 26, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 26, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1038605, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_006_002", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "260.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 62968.584, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-C_026.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 121, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-C", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:02:10 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 3, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -74, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-3-27": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 12, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 260, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "b9c94639-31ca-487c-8592-2e8628c3fb6d", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 20, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:01:37 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 20, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "725/40", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 27, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 27, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1038954, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_006_002", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "260.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 62968.584, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-C_027.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 122, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-C", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:02:10 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 3, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -74, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-3-28": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 13, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 260, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "00056107-e2b6-4954-b803-4da01244f6e3", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 20, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:01:37 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 20, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "725/40", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 28, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 28, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1039302, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_006_002", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "260.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 62968.584, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-C_028.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 123, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-C", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:02:11 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 3, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -74, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-3-29": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 14, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 260, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "671535c2-b645-48c5-b3f6-b4ccfbfc7fa5", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 20, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:01:37 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 20, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "725/40", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 29, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 29, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1039653, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_006_002", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "260.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 62968.584, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-C_029.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 124, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-C", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:02:11 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 3, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -74, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-3-30": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 15, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 260, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "be6a844f-b208-4092-a82f-fdef3b5675b3", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 20, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:01:37 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 20, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "725/40", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 30, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 30, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1040002, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_006_002", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "260.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 62968.584, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-C_030.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 125, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-C", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:02:11 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 3, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -74, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-4-0": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -15, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 10, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "1bbfe6a3-acf6-4dbc-b29c-a3ca62aaa14b", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 20, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:01:37 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 20, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "647/57", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 0, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 0, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "LudlShutter", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1041113, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_006_002", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "10.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 62968.584, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_P-DIC_000.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 95, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "P-DIC", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:02:13 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 4, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -74, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-4-1": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -14, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 10, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "eca84d61-6550-4f7e-be5c-e54caa90460e", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 20, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:01:37 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 20, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "647/57", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 1, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 1, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "LudlShutter", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1041528, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_006_002", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "10.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 62968.584, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_P-DIC_001.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 96, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "P-DIC", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:02:13 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 4, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -74, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-4-2": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -13, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 10, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "8135d7bc-c92b-4c2d-ba53-a4d17008e362", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 20, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:01:37 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 20, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "647/57", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 2, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 2, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "LudlShutter", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1041961, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_006_002", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "10.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 62968.584, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_P-DIC_002.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 97, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "P-DIC", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:02:13 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 4, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -74, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-4-3": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -12, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 10, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "762d03aa-e84d-4265-a334-a8a3e7564d70", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 20, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:01:37 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 20, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "647/57", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 3, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 3, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "LudlShutter", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1042394, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_006_002", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "10.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 62968.584, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_P-DIC_003.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 98, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "P-DIC", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:02:14 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 4, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -74, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-4-4": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -11, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 10, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "64bf5a2f-00a0-44be-9a7e-40a54af05e90", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 20, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:01:37 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 20, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "647/57", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 4, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 4, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "LudlShutter", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1042808, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_006_002", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "10.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 62968.584, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_P-DIC_004.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 99, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "P-DIC", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:02:14 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 4, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -74, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-4-5": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -10, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 10, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "fd648069-aaeb-44ad-a5ef-d919002be1c5", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 20, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:01:37 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 20, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "647/57", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 5, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 5, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "LudlShutter", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1043224, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_006_002", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "10.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 62968.584, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_P-DIC_005.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 100, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "P-DIC", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:02:15 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 4, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -74, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-4-6": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -9, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 10, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "d480f283-ebac-487e-9890-657b98a28b94", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 20, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:01:37 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 20, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "647/57", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 6, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 6, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "LudlShutter", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1043658, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_006_002", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "10.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 62968.584, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_P-DIC_006.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 101, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "P-DIC", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:02:15 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 4, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -74, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-4-7": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -8, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 10, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "4f8a829b-f245-485a-84e4-d4d421ae7155", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 20, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:01:37 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 20, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "647/57", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 7, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 7, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "LudlShutter", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1044103, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_006_002", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "10.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 62968.584, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_P-DIC_007.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 102, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "P-DIC", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:02:16 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 4, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -74, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-4-8": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -7, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 10, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "648b6b53-3ba8-4967-a5da-e79f1c42b089", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 20, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:01:37 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 20, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "647/57", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 8, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 8, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "LudlShutter", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1044520, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_006_002", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "10.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 62968.584, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_P-DIC_008.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 103, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "P-DIC", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:02:16 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 4, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -74, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-4-9": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -6, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 10, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "5a7694db-a83e-44a1-a249-6d31f38cd485", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 20, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:01:37 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 20, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "647/57", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 9, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 9, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "LudlShutter", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1044969, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_006_002", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "10.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 62968.584, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_P-DIC_009.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 104, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "P-DIC", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:02:16 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 4, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -74, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-4-10": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -5, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 10, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "68acbd50-9f1f-476f-86b0-38c706b453a0", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 20, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:01:37 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 20, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "647/57", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 10, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 10, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "LudlShutter", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1045414, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_006_002", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "10.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 62968.584, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_P-DIC_010.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 105, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "P-DIC", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:02:17 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 4, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -74, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-4-11": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -4, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 10, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "e4ab17b2-dc5b-472e-af70-640b5ad15886", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 20, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:01:37 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 20, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "647/57", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 11, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 11, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "LudlShutter", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1045849, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_006_002", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "10.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 62968.584, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_P-DIC_011.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 106, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "P-DIC", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:02:17 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 4, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -74, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-4-12": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -3, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 10, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "d6471cff-946e-426b-bd4c-039dd080fbe3", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 20, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:01:37 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 20, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "647/57", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 12, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 12, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "LudlShutter", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1046263, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_006_002", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "10.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 62968.584, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_P-DIC_012.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 107, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "P-DIC", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:02:18 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 4, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -74, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-4-13": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -2, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 10, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "9be344cd-785a-4a40-bb88-c579c3714313", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 20, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:01:37 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 20, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "647/57", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 13, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 13, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "LudlShutter", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1046679, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_006_002", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "10.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 62968.584, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_P-DIC_013.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 108, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "P-DIC", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:02:18 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 4, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -74, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-4-14": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -1, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 10, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "6a4d7d67-72cb-4e09-9b1e-fecb551c6bad", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 20, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:01:37 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 20, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "647/57", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 14, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 14, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "LudlShutter", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1047112, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_006_002", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "10.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 62968.584, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_P-DIC_014.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 109, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "P-DIC", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:02:19 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 4, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -74, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-4-15": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 0, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 10, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "dd3cc7fa-b2a5-4f14-bad1-4b39761ff585", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 20, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:01:37 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 20, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "647/57", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 15, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 15, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "LudlShutter", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1047527, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_006_002", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "10.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 62968.584, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_P-DIC_015.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 110, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "P-DIC", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:02:19 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 4, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -74, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-4-16": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 1, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 10, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "7290649c-3e2a-459a-8f33-f43faf204b1e", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 20, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:01:37 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 20, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "647/57", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 16, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 16, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "LudlShutter", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1047960, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_006_002", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "10.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 62968.584, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_P-DIC_016.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 111, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "P-DIC", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:02:19 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 4, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -74, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-4-17": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 2, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 10, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "d9d411fe-00e0-47e6-b6fd-72d578d56dc7", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 20, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:01:37 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 20, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "647/57", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 17, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 17, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "LudlShutter", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1048375, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_006_002", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "10.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 62968.584, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_P-DIC_017.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 112, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "P-DIC", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:02:20 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 4, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -74, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-4-18": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 3, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 10, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "2470dd82-37ad-434e-ab5f-39b808dc15ec", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 20, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:01:37 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 20, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "647/57", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 18, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 18, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "LudlShutter", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1048808, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_006_002", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "10.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 62968.584, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_P-DIC_018.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 113, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "P-DIC", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:02:20 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 4, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -74, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-4-19": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 4, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 10, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "bfbb144f-c015-4770-90a4-6db7a3c759dc", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 20, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:01:37 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 20, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "647/57", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 19, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 19, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "LudlShutter", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1049224, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_006_002", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "10.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 62968.584, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_P-DIC_019.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 114, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "P-DIC", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:02:21 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 4, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -74, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-4-20": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 5, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 10, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "229256b4-3e32-4a2e-905f-c669c0e5448c", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 20, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:01:37 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 20, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "647/57", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 20, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 20, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "LudlShutter", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1049655, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_006_002", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "10.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 62968.584, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_P-DIC_020.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 115, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "P-DIC", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:02:21 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 4, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -74, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-4-21": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 6, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 10, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "f20a2e47-d2ef-4904-ae39-a5bc679225e4", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 20, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:01:37 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 20, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "647/57", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 21, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 21, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "LudlShutter", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1050088, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_006_002", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "10.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 62968.584, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_P-DIC_021.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 116, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "P-DIC", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:02:22 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 4, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -74, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-4-22": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 7, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 10, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "7d62b310-599b-4fed-a073-2185cbd6ddb8", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 20, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:01:37 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 20, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "647/57", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 22, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 22, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "LudlShutter", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1050518, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_006_002", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "10.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 62968.584, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_P-DIC_022.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 117, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "P-DIC", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:02:22 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 4, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -74, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-4-23": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 8, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 10, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "b23993f0-fbde-4ff6-8de8-bd9dd08efc19", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 20, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:01:37 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 20, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "647/57", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 23, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 23, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "LudlShutter", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1050950, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_006_002", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "10.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 62968.584, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_P-DIC_023.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 118, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "P-DIC", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:02:22 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 4, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -74, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-4-24": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 9, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 10, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "72a89a4e-5dc0-4833-896b-b8209241b801", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 20, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:01:37 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 20, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "647/57", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 24, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 24, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "LudlShutter", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1051398, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_006_002", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "10.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 62968.584, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_P-DIC_024.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 119, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "P-DIC", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:02:23 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 4, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -74, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-4-25": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 10, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 10, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "09f8829d-5e26-4c29-9e1f-95d0e48b210f", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 20, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:01:37 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 20, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "647/57", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 25, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 25, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "LudlShutter", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1051830, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_006_002", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "10.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 62968.584, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_P-DIC_025.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 120, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "P-DIC", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:02:23 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 4, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -74, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-4-26": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 11, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 10, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "9de2d6e5-396d-48ea-a1e4-cec6ac11b8af", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 20, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:01:37 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 20, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "647/57", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 26, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 26, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "LudlShutter", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1052279, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_006_002", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "10.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 62968.584, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_P-DIC_026.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 121, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "P-DIC", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:02:24 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 4, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -74, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-4-27": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 12, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 10, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "1f610e38-5599-4484-9677-e2d5e79dae88", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 20, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:01:37 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 20, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "647/57", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 27, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 27, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "LudlShutter", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1052711, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_006_002", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "10.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 62968.584, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_P-DIC_027.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 122, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "P-DIC", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:02:24 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 4, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -74, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-4-28": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 13, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 10, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "acb4bc19-2165-42cc-9317-f013aa5d55fc", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 20, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:01:37 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 20, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "647/57", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 28, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 28, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "LudlShutter", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1053126, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_006_002", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "10.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 62968.584, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_P-DIC_028.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 123, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "P-DIC", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:02:25 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 4, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -74, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-4-29": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 14, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 10, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "a3a07f79-599a-461b-a7dd-ffa44e54ce39", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 20, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:01:37 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 20, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "647/57", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 29, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 29, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "LudlShutter", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1053574, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_006_002", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "10.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 62968.584, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_P-DIC_029.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 124, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "P-DIC", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:02:25 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 4, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -74, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-4-30": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 15, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 10, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "cfd3dfd2-a6bb-4d77-b0d8-151bd193d2a7", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 20, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:01:37 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 20, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "647/57", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 30, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 30, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "LudlShutter", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1054023, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_006_002", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "10.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 62968.584, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_P-DIC_030.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 125, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "P-DIC", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:02:26 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 4, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -74, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" } }